About the Harris Research Program

 

Professor Eva Harris has developed a multidisciplinary approach to study the molecular virology, pathogenesis, immunology, epidemiology, clinical aspects, and control of dengue, Zika, and chikungunya—the most prevalent mosquito-borne diseases in humans.

Our program investigates viral and host factors that modulate disease severity and immune correlates of protection and pathogenesis, using in vitro approaches, animal models, and research involving human populations.

 

Projects

One major focus of the Harris Research Program is on studies of arboviral disease in humans, including:

  1. Antibody and B cell responses and correlates of protection

  2. Systems immunology profiling of the innate response

  3. Viral evolution, fitness, and intra-host diversity

  4. Viral pathogenesis: the role of non-structural 1 (NS1) protein in vascular leak

Professor Harris’ international work focuses on laboratory-based and epidemiological studies of dengue, Zika, chikungunya, and influenza in endemic Latin American countries, particularly in Nicaragua, where she has been working closely with the Ministry of Health for over 30 years. Long-term collaborations include clinical, biological, and immunological studies of severe disease through a 20-year pediatric hospital-based study; a 15-year ongoing pediatric cohort study of dengue, Zika, chikungunya, and influenza transmission in Managua; and a cluster randomized controlled trial of evidence-based community-derived interventions to prevent and control arboviral diseases.

Featured Publications

Seroprevalence, risk factor, and spatial analysis of Zika virus infection after the 2016 epidemic in Managua, Nicaragua. 
Zambrana, J.V., Bustos, F., Burger-Calderon, R., Collado, D., Jairo, Sanchez, N., Ojeda, S., Plazaola, M., Lopez, B., Arguello, S., Elizondo, D., Aviles, W., Kuan, G., Balmaseda, A., Gordon, A., Harris, E. (2018)
Proc. Natl. Acad. Sci. USA. 2018 Sep 11;115(37):9294-9299. doi: 10.1073/pnas.1804672115. Epub 2018 Aug 27.

Comprehensive innate immune profiling of chikungunya virus infection in pediatric cases.
Michlmayr D., Pak T.R., Rahman A.H., Amir E.D., Kim E.Y., Kim-Schulze S., Suprun M., Stewart M.G., Thomas G.P., Balmaseda A., Wang L., Zhu J., Suaréz-Fariñas M., Wolinsky S.M., Kasarskis A., Harris E. (2018)
Mol. Syst. Biol. 2018 Aug 27;14(8):e7862. doi: 10.15252/msb.20177862.

Differences in transmission and disease severity between two successive waves of chikungunya.
Gordon, A., Gresh, L., Ojeda, S., Chowell-Puente, G., Gonzalez, K., Sanchez, N., Saborio, S., Mercado, J.C., Kuan, G., Balmaseda, A., and Harris, E. (2018)
Clin. Infect. Dis. 2018 Apr 25. doi: 10.1093/cid/ciy356. [Epub ahead of print].

Antibody-dependent enhancement of severe dengue disease in humans.
Katzelnick., L. Gresh, L, Halloran, M.E., Mercado, J.C., Kuan, G., Gordon, A., Balmaseda, A., and Harris, E. (2017)
Science. 358(6365):929-932.

Longitudinal analysis of antibody cross-neutralization following Zika and dengue virus infection in Asia and the Americas
Montoya, M., Collins, M., Dejnirattisai, W., Katzelnick, L.C., Puerta-Guardo, H., Jadi, R., Schildhauer, S., Supasa, P., Vasanawathana, S., Malasit, P., Mongkolsapaya, J., de Silva, A.D., Tissera, H., Balmaseda, A., Screaton, G., de Silva, A.M., Harris, E. (2018)
J. Infect. Dis. 218(4):536-545.

CD14+ CD16+ monocytes are the main targets of Zika virus infection in peripheral blood mononuclear cells in a paediatric study in Nicaragua.
Michlmayr, D., Andrade, P., Gonzalez, K., Balmaseda, A., and Harris E. (2017)
Nat Microbiol. 2(11):1462-1470. 2017.

Dengue virus NS1 cytokine-independent vascular leak is dependent on endothelial glycocalyx integrity. 
Glasner, D.R., Ratnasiri, K., Puerta-Guardo, H., Beatty, P.R., and Harris, E. (2017)
PLoS Pathog. 13(11):e1006673.

Analysis of individuals from a dengue-endemic region helps define the footprint and repertoire of antibodies targeting dengue virus 3 type-specific epitopes.
Andrade, D.V., Katzelnick, L.C., Widman, D.G., Balmaseda, A., de Silva A.M., Baric, R.S., and Harris, E. (2017)
mBio. 8(5):e01205-17.

Intrahost selection pressures drive rapid dengue virus microevolution in acute human infections.
Parameswaran, P., Wang, C., Trivedi, S.B., Eswarappa, M., Montoya, M., Balmaseda, A., and Harris, E. (2017)  
Cell Host Microbe. 22(3):400-410.e5.

Zika virus targets different primary human placental cells, suggesting two routes for vertical transmission. 
Tabata, T., Petitt, M., Puerta-Guardo, H., Michlmayr, D., Wang, C., Fang-Hoover, J., Harris, E.* and Pereira, L.* (2016)
Cell Host Microbe. In press (Epub July 18, 2016) S1931-3128(16)30300-6. doi: 10.1016/j.chom.2016.07.002. *Co-corresponding authors. 

Dengue virus NS1 disrupts the endothelial glycocalyx, leading to hyperpermeability. 
Puerta-Guardo, H., Glasner, D.R., and Harris, E. (2016)
PLoS Pathog.12(7):e1005738.

Dengue virus non-structural protein 1 (NS1) triggers vascular leak that can be inhibited by anti-NS1 antibodies. 
Beatty, P.R., Puerta Guardo, H., Killingbeck, S., Glasner, D., Hopkins, K., and Harris, E. (2015)
Sci Transl Med. 7:304ra141.

Evidence based community mobilization for dengue prevention in Nicaragua and Mexico (Camino Verde, the Green Way): cluster randomized controlled trial.
Andersson, N., Nava-Aguilera, E., Arostegui, J., Morales-Perez, A., Suazo-Laguna, H., Legorreta-Soberanis, J., Hernandez-Alvarez, C., Fernandez-Salas, I., Balmaseda, A., Cortés-Guzmán, A.J., Coloma, J., Ledogar, R.J., and Harris, E. (2015)  
BMJ. 351:h3267.

Contact Us

To reach the Harris Research Program, email Dr. Eva Harris at eharris (at) berkeley (dot) edu

 

Address:
510D Li Ka Shing Center
University of California
1951 Oxford Street
Berkeley, CA 94720-3370